Zahlavi

Selection Procedures

10/14/2021
Institute of Biotechnology of the CAS announces an open competition in accordance with the Act No. 283/1992 Coll. on the Czech Academy of Sciences, and the Statutes of the Czech Academy of Sciences for a position Ph.D. Student position: Mitochondria and Cancer.

Laboratory of Tumour Resistance of the Institute of Biotechnology of the Czech Academy of Sciences announces a selection procedure in accordance with the Act No. 283/1992 Coll. on the Czech Academy of Sciences, and the Statutes of the Czech Academy of Sciences for a Ph.D. student for the research project Molecular Mechanisms of Cancer Resistance: The Role of Mitochondria".

The project will address the role of mitochondrial function and metabolism and its impact on cancer resistance. It is clear that these organelles are vital in the process of carcinogenesis, but is becoming evident that they could also participate in the resistance to treatment.

The main aim of this project is to delineate the role of metabolic alterations coupled with mitochondria and their participation in the development or maintenance of cancer resistance. The role of experimental targets, which affect mitochondrial function and metabolism, will be tested by gain of function (overexpression) and loss of function (knockout, CRISPR/Cas system) studies. Genetically modified cell lines will be tested by live cell imaging using Etaluma720, enabling simultateous determination of viable and dead cells in real time. We will then assess the impact of such modifications on resistance of these cell lines to cell death induction. We also plan to investigate the role of post-translational regulatory sites of the prospective targets governing the mitochondrial metabolism, this will be assesed by site-directed mutagenesis, that would either result in loss of function or lead to a constitutively active or inactive mutants.

The experimental model that will primarily be explored is a model of tamoxifen resistant breast cancer cells, due to its high clinical relevance and our previous characterization of its particular metabolic phenotype (DOI:10.1016/j.freeradbiomed.2019.09.004).

Requirements:

  • Finished Master degree in Biochemistry or Molecular/ Developmental/ Cellular Biology
  • Good communication skills in spoken and written English
  • Motivation to actively participate in this project
  • Ability to work in a team

What we offer:

  • Position of a Ph.D., grant support, interesting topic
  • Participation in international conferences
  • Laboratory with modern equipment, in the newly built BIOCEV center in Vestec
  • Small but enthusiastic team

For more information about the Laboratory members and PI, see the web page of the Laboratory of Tumour Resistance: https://www.ibt.cas.cz/en/research/laboratory-of-tumour-resistance or Research Gate profile https://www.researchgate.net/profile/Jaroslav-Truksa

In case you are interested, please send your application with a structured CV to jaroslav.truksa@ibt.cas.cz.

Deadline for applications: 30. 11. 2021

More details on the processing of personal data are available on the website: https://www.ibt.cas.cz/cs/verejnost-media/oficialni-dokumenty/gdpr/.

DOI: 10.1016/j.cell.2016.07.0020.1016


Application deadline: 11/30/2021